We have described the design of our triple γ-detection system, and its use for pharmacokinetic characterization of radiotracers in the Langendorff perfused heart previously23,24. Briefly, it comprises three orthogonal lead-collimated Na/I γ-detectors arrayed around a perfusion apparatus, positioned (i) 3 cm downstream of a radiotracer injection port on the arterial line, 15 cm upstream of the heart cannula (to provide a radiotracer input function), (ii) directly opposite the heart (to monitor cardiac retention), and (iii) over the venous outflow line (to provide an output function). Each detector was connected to a modified GinaSTAR™ ITLC system running Gina™ software for real-time data collection (Raytest Ltd, UK).
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