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4.5. Cytosolic, Nuclear, and Enriched Plasma Membrane Extracts

DM Danila Di Meo
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Cytosolic and nuclear fractions were isolated from resting and PHA-stimulated Jurkat cells [17] and from SH-SY5Y cells treated for 24 h with curcumin and C1, or after starvation, as previously described. Plasma membrane proteins were isolated from soluble proteins using the Mem-PER Eukaryotic Membrane Protein Extraction Kit (Pierce, Thermo Scientific, Waltham, MA, USA) in accordance with the manufacturer’s procedure. Protein concentration was determined by Bradford’s assay.

Copyright and License information:  ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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