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Intracerebroventricular Cannulation and Administration
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The intracerebroventricular (i.c.v.) injections were carried out as previously described 37. The rats recovered from surgery for 7 days before being injected with 5 µl of either vehicle saline control or 4 μM L‐Arg (n = 8–9) over 1 min using a 28‐gauge stainless steel injector in the early light phase.
Copyright and License information: ©2016 The Authors. Diabetes, Obesity and Metabolism published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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