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Mice were intraperitoneally injected with 100μl of 7.5 mg/ml D-luciferin (Xenogen), and after 10 minutes they were anaesthetized with isoflurane inhalation. And then Bioluminescence imaging with a CCD camera (IVIS, Xenogen) was used for living imaging with automatic exposure time. Flurorescence emission was normalized to photons per second per centimeter squared per steradian (p/s/cm2/sr) and the final imaging results of three weeks were unified to the same scale size.
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