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PCR assay for microsatellite instability
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Genomic DNA was extracted and PCR-amplified at five MSI markers (Buhard et al., 2004; Buhard et al., 2006). PCR primers are from Buhard et al. (2006), with one primer in each pair labeled with a fluorescent dye for combined analysis. PCR products were run on a Genetic Analyzer ABI 3730 to produce fragment profiles, which were visualized using Peak Scanner version 1.0 (ThermoFisher).
Copyright and License information: ©2019, Hung et al
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