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2.7.1. Plasma and serum analyses

JJ Jamie N. Justice
AN Anoop M. Nambiar
TT Tamar Tchkonia
NL Nathan K. LeBrasseur
RP Rodolfo Pascual
SH Shahrukh K. Hashmi
LP Larissa Prata
MM Michal M. Masternak
SK Stephen B. Kritchevsky
NM Nicolas Musi
JK James L. Kirkland
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Plasma cytokines were quantified using multiplex ELISA on a Bio-Plex 200 analyzer by Eve Technologies (Calgary, Alberta, Canada) using Human Cytokine 42-plex, MMP9 plex, and TIMP 4-plex discovery assays. All samples were run in duplicates. Single targeted ELISA's (vendors below) were run to measure plasma osteopontin, apelin12, PAI-1, activin A, IL-6, and MCP-1, and serum MMP-1 and MMP-7 (Human Activin A: DAC008, R&D systems; Human Total MMP-7 [serum]: DMP700, R&D systems; Human IL-6: D6050, R&D systems; Human Serpin E1/PAI-1: DSE100, R&D systems; Human CCL2/MCP-1: DCP00, R&D systems; Human Osteopontin: BMS2066, eBioscience; Human Apelin-12: EK-057-23, Phoenix Pharmaceuticals; Human MMP-1 (serum): ELH-MMP-1, RayBiotech., Inc.).

Copyright and License information:  ©2018 The Authors
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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