Evaluation of Cold Tolerance in a Line Over-Expressing OsWYRKY71

MK Manu Kumar
YG Yun-Shil Gho
KJ Ki-Hong Jung
SK Seong-Ryong Kim
request Request a Protocol
ask Ask a question
Favorite

Plants from an Ox line for OsWYRKY71 (OsWYRKY71-Ox) under the control of CaMV35S promoter (Kim et al., 2016) and from the WT (Japonica cv. Dongjin) were grown for 10 days in plastic boxes containing soil. To test their tolerance, we then exposed them to cold stress (4oC) for 5 days and then returned them to normal growing conditions for 6 days of recovery. Survival rates were determined at the end of this experimental period. Cold stress analysis of OsWYRKY71-Ox lines was done with three replicates.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A