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NR4A1 Reporter Gene Assay

JK Ji In Kang
YC Yoonjung Choi
CC Chang-Hau Cui
DL Daeyoup Lee
SK Sun Chang Kim
HK Ho Min Kim
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HEK293T cells were transfected with the NR4A1 response element (NurRE) luciferase reporter construct which contains 3 copies of NR4A1 binding sites from the POMC gene promoter37, and 10 μM of ginsenosides (F1 or Rh1) were treated for 1 hr. For the positive control (stimulation of transcription activity of NR4A1), 0.1 μM of Csn-B (Cat#C2997, Sigma-Aldrich), which is a fungal metabolite closely related to phomposin C and the naturally occurring agonist for NR4A1 with high affinity (IC50 = 0.278 nM)38, was co-treated. Then, the dual luciferase reporter assay (Cat#E1910, Promega) was performed by following the manufacturer’s instruction.

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