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Preparation of antibody-coated dynabeads

JW Jinping Wang
NN Niu Niu
SX Suowen Xu
ZJ Zheng Gen Jin
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The day before cell harvest,100 µl dynabeads was incubated with 10 µl CD31 antibody (CD31-coated beads, for first sorting) and 10 µl ICAM2 antibody (ICAM2-coated beads, for second sorting), respectively, under constant stirring at 4 degree overnight. Then, the suspension was placed on magnetic rack and the supernatant as aspirated, and the bound beads were washed three times with beads washing solution. After each wash, the coated beads are placed on the magnetic rack for 1 min. Supernatant was carefully aspirated without disturbing the beads. After the final wash, the antibody-coated dynabeads were suspended with same volume of beads washing solution (100 µl) and stored at 4 degree for use.

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