Protein lysate preparation and western blot analysis

Cells were lysed in RIPA buffer containing 150 mM NaCl, 50 mM Tris pH 8.0, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS supplemented with protease inhibitors (Complete, Roche) and phosphatase inhibitor cocktails II and III (Sigma) and boiled for 10 min after addition of sample buffer (60 mM Tris pH 6.8, 5% glycerol, 1% SDS, 2% β-mercaptoethanol, 0.02% bromophenol blue) before SDS gel electrophoresis followed by western blotting. Primary antibody against HSP90 (SC-7947) was purchased from Santa Cruz. Antibodies against acetyl CoA carboxylase 1 (#4190), beta-actin (#4967), cl-PARP (#9542), p-AKT (#4060), p-PRAS40 (#2997), p-S6 (#2211), p-4EBP1 (#9456) and p-BAD (#5284) were from Cell Signaling. Secondary antibodies were obtained from Bio-Rad Laboratories and Thermo Scientific. Uncropped blots are shown in Supplementary Fig. 7.