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Rapamycin (Calbiochem) was dissolved in dimethyl sulfoxide (DMSO; 5 mg/125 μL) and then diluted in PBS (1: 100 v/v). The total volume was aliquoted in 1 mL and frozen at −20°C until use. Three ad libitum fed BALB/c male mice received every 48 h for 20 days an intraperitoneal injection of 100 μL (2 mg/ kg) of the drug. Other three individuals were used as sham group (DMSO vehicle injection).
Copyright and License information: ©2019 Peña-Villalobos, Casanova-Maldonado, Lois, Sabat and Palma.
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