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CdpR proteins were mixed with DNA probes (S4 Table) in 20 μl of the gel shift-loading buffer (20 mM HEPES, pH 8.0, 100 mM NaCl, 0.5 mM dithiothreitol, 10% Glycerol, and 3 μg/ml sheared salmon sperm DNA). After incubation at room temperature for 20 min, the samples were analyzed by 6% polyacrylamide gel electrophoresis in 0.5×TBE (Tris/Boric Acid/EDTA) buffer at 90 V for 90 min. The gels were stained by SYBR GOLD dye and subjected to screen on a phosphor screen (Tanon 5500).
Copyright and License information: ©2016 Zhao et al
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
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