Influence of time and N treatment on N and C accumulation, growth (biomass DW), protein, TFA, PUFA, MUFA, chlorophyll a, and carotenoid, contents were analyzed using repeated measures ANOVA. Tukey’s test was used as a post hoc test. Because of heterogeneity in variances (Levene statistics, test of homogeneity of variances), statistics for SAFA and LC-PUFA contents were performed with the non-parametric Friedman’s test. A significance level of P < 0.05 was used in all tests. Results from non-parametric tests were Bonferroni corrected. All statistical analyses were done with SPSS (Version 24; IBM, New York City, NY, USA).
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