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The comparison of RmPAP expression among different tick tissues was performed using the Kruskal-Wallis test with Bonferroni’s multiple comparison post hoc test45. The comparison between partially and fully fed ticks was performed using the Mann-Whitney test. RmPAP knockdown was analyzed with Mann-Whitney test and the biological parameters were analyzed using Student’s two-tailed t-test. Analyses were conducted with the Graph Pad Prism 6.0 software (GraphPad Software, Inc.), and differences were considered to be statistically significant when p < 0.05.
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