Complementation tests with the other loss-of-function allele of hpo-30 showed non-complementation between hpo-30(dz178) and hpo-30(dz189). The mutation was further confirmed by Sanger sequencing of the original isolate, identifying a non-sense mutation C to T mutation in hpo-30(dz189) (see List of hpo-30 mutant alleles below).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.