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SL S. H. Lee
CM C. A. Mastronardi
RL R. W. Li
GP G. Paz-Filho
ED E. G. Dutcher
ML M. D. Lewis
AV A. D. Vincent
PS P. N. Smith
SB S. R. Bornstein
JL J. Licinio
MW M. L. Wong
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Piecewise non-linear mixed effects growth curve models were constructed to estimate rat weights, y, post-stress over two periods: (A) days 20–60 and (B) day 60 onward. Analyses performed in R v3.4.1 (Vienna, Austria) using the nlme package (see supplementary methods for detailed statistical analysis)19.

Group comparisons were done by one-way analysis of variance (ANOVA) or non-parametric test of Kruskal-Wallis when the variances were not equal among treatment groups, and respectively followed by Tukey’s or Dunn’s post hoc test, using the GraphPad Prism 5.0 software (La Jolla, CA, USA) (see supplementary methods for detailed statistical analysis).

Associations between the five body composition outcomes [bone mineral content (BMC), bone mirenal density (BMD), % fat, body length, body weight) with four biochemical measures (log-transformed plasma leptin, total cholesterol, triglyceride, and fatty acids levels) were tested using linear regressions (see supplementary methods for detailed statistical analysis).

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