Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Sequencing of amplified PCR products

PS Pedro Seoane
ST Silvana T. Tapia-Paniagua
RB Rocío Bautista
EA Elena Alcaide
CE Consuelo Esteve
EM Eduardo Martínez-Manzanares
MB M. Carmen Balebona
MC M. Gonzalo Claros
MM Miguel A. Moriñigo
request Request a Protocol
ask Ask a question
Favorite

DNA from amplicons was purified using GeneJET PCR Purification Kit (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions and then sequenced at Macrogen (Seul, South Korea). The quality control of the sequences was performed using the percentage of bases with a quality score higher than 20 (reported by Macrogen). Then, the sequences were compared with the complete genome of Pdp11 using BLASTN (Camacho et al., 2009).

Copyright and License information:  ©2019 Seoane et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A