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Sirius Red staining

LM Lindsey A. Muir
CN Christopher K. Neeley
KM Kevin A. Meyer
NB Nicki A. Baker
AB Alice M. Brosius
AW Alexandra R. Washabaugh
OV Oliver A. Varban
JF Jonathan F. Finks
BZ Brian F. Zamarron
CF Carmen G. Flesher
JC Joshua S. Chang
JD Jennifer B. DelProposto
LG Lynn Geletka
GM Gabriel Martinez-Santibanez
NK Niko Kaciroti
CL Carey N. Lumeng
RO Robert W. O'Rourke
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Slides were deparaffinized in xylene/ethanol, stained with Sirius Red/Fast Green dye (Chondrex Inc., Redmond, WA, USA) and visualized on an Olympus BX-51 inverted microscope with bright-field and plane-polarized light. Staining intensities of multiple 10× fields of identical size were analyzed from multiple slides from each sample, and staining normalized to tissue area i.e. total number of fields analyzed, quantified with ImageJ software, and averaged for each patient.

Copyright and License information:  ©2016

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