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The automated DNA extraction was performed on 190 μl collected samples from skin swabs, blood, lice-washing liquid, crushed lice using a BioRobot®EZ1 Advanced XL instrument (QIAGEN, Hilden, Germany) and DNeasy® Blood & Tissue according to the manufacturer's instructions. The quality of all DNA extracts was assessed by real-time PCR (qPCR) targeting internal control TISS phage that was added to each extraction (Sow et al., 2017).
Copyright and License information: ©2019 Ly, Kerbaj, Edouard, Hoang, Louni, Dao, Benkouiten, Badiaga, Tissot-Dupont, Raoult, Brouqui, Mediannikov and Gautret.
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