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GST-tagged proteins were expressed in E. coli BL21 and purified under native conditions from bacterial cell lysate by affinity chromatography using columns with Pierce Glutathione agarose (Thermo Fisher Scientific) and performed as recommended by the manufacturer. Proteins were eluted with reduced glutathione (Roth). Protein concentration was calculated by Bradford assay (Bio Rad) and verified using SDS/PAGE coupled with coomassie staining.
Copyright and License information: ©2019 Braun et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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