Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Plant material

EF Edith Forestier
CR Carmen Romero-Segura
IP Irini Pateraki
EC Emilio Centeno
VC Vincent Compagnon
MP Myriam Preiss
AB Anne Berna
AB Albert Boronat
TB Thomas J. Bach
SD Sylvain Darnet
HS Hubert Schaller
request Request a Protocol
ask Ask a question
Favorite

Euphorbia lathyris L. plants were grown in a growth chamber under a long-day light regime at 24 °C during the light phase (16 h) and 20 °C during the dark phase (8 h). Seeds were germinated in standard garden soil for one month then seedlings of 20–30 cm in height were transferred into individual pots. Latex samples were collected through razor-blade transversal sections of stems starting from the apices to bottoms and every 10 cm to take advantage of the pressure in laticifers to drain the tissues as much as possible. Nicotiana benthamiana Domin. plants were grown in a standard soil mixture (Archut Fruhstorfer Erde®) in a growth chamber maintained under a long-day light regime (16 h light, 120 μmol photons m−2 sec−1).

Copyright and License information: The Author(s) ©2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A