DNA extraction

VH Verena Hammerl
EK Eva-Maria Kastl
MS Michael Schloter
SK Susanne Kublik
HS Holger Schmidt
GW Gerhard Welzl
AJ Anke Jentsch
CB Carl Beierkuhnlein
SG Silvia Gschwendtner
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DNA was extracted from 0.5 g fresh soil using the FastDNA® SPIN Kit for Soil (MP Biomedicals, Canada) and the Precellys24 Instrument (Bertin Technologies, France) according to the manufacturer’s protocol. After extraction, DNA quantity and quality were determined using the spectrophotometer Nanodrop (PeqLab, Germany) (73–214 ng µL−1 DNA). Subsequently, DNA was stored at −20 °C until further processing.

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