Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
For apoptosis analysis, 5 × 105 cells (including RK13, Hela, and HepG2 cells) were seeded onto plates and transfected with recombinant plasmids (including pcDNA3.1-NSP6 and mutants) or empty vector for 24 h. The cells were then washed with PBS and resuspended in 100 μL binding buffer supplemented with 5 μL annexin V (10 μg/mL) and 5 μL propidium iodide (PI) (20 μg/mg) for 10 min in the dark. Next, 400 μL binding buffer was added, and the samples were immediately screened using flow cytometry to assess annexin V staining and permeability.
Copyright and License information: ©2019 Chen, Liu, Hu, Fan, Song, Wei, Qiu, Xu, Zhu and Wang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
