Cell transfection

A431 and SCL-1 cells in the logarithmic growth phase were inoculated into cell culture plates according to the appropriate cell numbers and cultured overnight. The following day, the cells were treated with different concentrations (0, 0.4 and 0.8 mM) of crocin to detect the cell phenotypes. Alternatively, according to the instructions of Lipofectamine 2000, the cells were transfected with Jak2 overexpression plasmid (pcDNA-Jak2) for 8 h, and then replaced with complete medium for further culture. The related phenotypes were detected after the transfection process.