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DNA purification

DT Dóra Tombácz
ZM Zoltán Maróti
TK Tibor Kalmár
MP Miklós Palkovits
MS Michael Snyder
ZB Zsolt Boldogkői
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Genomic DNA (gDNA) samples were isolated from the occipital, cerebellar or somatomotor cortex regions (Table 1 (available online only)) using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s recommendations for the spin-column protocol, using 30 mg staring tissue material from each sample. In short, tissue samples were cut into small pieces, and then lysis buffer (provided by the kit) and proteinase K were added. Lysis reactions were carried out at 56°C until complete lysis was obtained. DNeasy Mini spin columns (kit’s component) were used for the isolation of gDNA from the lysate. Elution was carried out twice to a final volume of 100 μl per elution.

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