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Consensus from 16S rRNA gene coding sequences of the 273 isolates were aligned using MUSCLE algorithm (Edgar, 2004a,b). The phylogenetic analysis of 480 bp aligned sequences from the V2–V4 16S gene regions (Position: 201–681) was performed using MEGA V7 software (Kumar et al., 2016). Phylogeny was inferred by maximum likelihood, with 1000 bootstrap iterations to test the robustness of the nodes. The resulting tree was uploaded and formatted using iTOL (Letunic and Bork, 2016).
Copyright and License information: ©2019 Corre, Delafont, Legrand, Berjeaud and Verdon.
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