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Enrichment of regulatory T (Treg) cells
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LMC suspensions were obtained as previously was described from the liver of infected mice. Treg were enriched by using magnetic microbeads kit (CD4+ CD3+ Regulatory T cells isolation kit; Miltenyi Biotec) and AutoMACS Pro Separator (Miltenyi Biotec), following the provider instructions. First, CD4+ T cells were pre-enriched by depletion of unwanted cells, non-CD4+ T cells were indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and anti-biotin Microbeads. Then, CD25+ cells were positively selected from the enriched CD4+ T cells.
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