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The seedlings and organs of the adult pCOPT5::GUS and pNRAMP4::GUS plants were embedded with the substrate solution [100 mM NaPO4, pH 7.2, 0.5 mM K3Fe(CN)6, 0.5 mM K4Fe(CN)6, 0.1% (v/v) Triton X-100, 0.5 mM 5-bromo-4-chloro-3-indolyl-β-D glucuronide (AppliChem), and 10 mM EDTA, pH 7.2]51. Reactions took place at 37 °C and were stopped with ethanol (70%). The pictures of the cross sections were obtained using the Olympus AT70F microscope and the Infinity software.
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