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Migration inhibition assay

YW Ying Wang
YG Ying Nv Gao
RB Rui Bai
HC Hong Yu Chen
YW Ying Ying Wu
JS Jun Jun Shang
DB Da Peng Bao
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The wound-healing assay was used to compare the inhibitory effect of rLZ-8, rFIP-fve, and rFIP-bbo on the migration of the cells. The cells were cultured on 6-well plates and grown in a medium containing 10% FBS to a nearly confluent cell monolayer. A plastic pipette tip was used to draw a linear “wound” on the cell monolayer of each well. The monolayer was subsequently washed twice using PBS to remove debris or detached cells, and 8 μg/mL of rLZ-8, rFIP-fve and rFIP-bbo was added to the plates, respectively. PBS was added to the negative control. After 24 h and 36 h of incubation, the morphology of the cells was observed using an inverted microscope (Shanghai Cai Kang Optical Instrument Co., LTD, Shanghai, China), and captured by a digital camera coupled to the microscope (magnification, 200×).

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