Human neutrophil elastase activity and enzyme-linked immunosorbent assays (ELISAs)

JG Jocelyn R. Grunwell
VG Vincent D. Giacalone
SS Susan Stephenson
CM Camilla Margaroli
BD Brian S. Dobosh
MB Milton R. Brown
AF Anne M. Fitzpatrick
RT Rabindra Tirouvanziam
request Request a Protocol
ask Ask a question
Favorite

Human neutrophil elastase (HNE) activity was measured in ASN and plasma according to the supplier’s protocol (Cayman Chemical, Ann Arbor, MI), with a kit-supplied standard curve. In addition, human matrix metalloproteinase-9 (MMP-9) DuoSet, myeloperoxidase (MPO) Quantikine ELISA kits (R&D Systems, Minneapolis, MN), and human lactoferrin ELISA kit (Abcam, Cambridge, MA) were used according to suppliers’ protocols, and protein concentrations calculated using supplied standard curves. Data were collected on a BioTek (Winooski, VT) Synergy HT 96-well plate reader with recommended wavelengths and standard curve fit procedures.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A