Wound healing assay

Wound healing assays were performed to investigate the effect of miR-885-5p on cell migration. In brief, ~5×10⁵ transfected MG-63 or U2OS cells were seeded into 6-well plates. Once the cells had reached 90–100% confluence, a 20 µl pipette tip was used to scratch a line into the cell monolayer. Detached cells were removed by washing with PBS, and the cells were maintained in serum-free DMEM at 37°C. The relative distance of cell migration was measured via the width of the wound at 0 and 48 h following the scratch, under a light microscope (magnification, ×20). Each independent experiment was performed at least 3 times.