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Transepithelial electrical resistance (TEER) measurement
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1XPBS of 100 µl volume was added to the apical chamber of fully differentiated air-liquid interface cultures. Trans-epithelial electrical resistance (TEER) was then determined using the EVOM2 epithelial voltohmmeter (World Precision Instruments, FL, USA) equipped with the STX2 electrodes and a mean resistance was calculated. Values were then corrected for fluid resistance (insert with no cells) and surface area. All measurements were performed in triplicate.
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