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Manganese-enhanced MRI (MEMRI)

YM Yolandi van der Merwe
AF Anne E. Faust
ES Ecem T. Sakalli
CW Caroline C. Westrick
GH George Hussey
KC Kevin C. Chan
IC Ian P. Conner
VF Valeria L. N. Fu
SB Stephen F. Badylak
MS Michael B. Steketee
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Each animal (n = 4 per group) received a 1.5 µl bilateral injection of 100 mM MnCl2. T1-weighted MEMRI was done immediately before and then 8 hrs post injection with a fast spin echo imaging sequence as described35. Briefly, slices were oriented orthogonal to the pre-chiasmatic ON. Other imaging parameters include: TR/TE = 600/8 ms, echo train length = 8, number of slices = 8, and slice thickness = 1 mm. A saline phantom was placed next to the rodent head for normalization. Signal intensity values, from IOP-injured and uninjured animals, before (pre-MEMRI) and 8 hrs after (post-MEMRI) MnCl2 injections were calculated as percentage differences between the right and left signal intensities and compared between pre- and post-MEMRI to determine the percent decrease in intensity in the ON, LGN, and SC.

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