Tumor growth inhibition Assay in vivo

To evaluate tumor growth inhibition activity of CuHQTS in vivo, athymic nu/nu mice were implanted with PC-3 cells (5 × 10⁶/injection) subcutaneously in the left and right flanks. Three days after implantation of PC-3 cells, the athymic nu/nu mice implanted with PC-3 cells were treated with intra-peritoneal injection of CuHQTS (15 µg/g body weight, dissolved in100 µL saline containing 1% DMSO) every other day. Another group of athymic nu/nu mice implanted with PC-3 cells were treated with normal saline as a group of placebo control. Tumor growth in mice implanted with PC-3 cells was evaluated by visual assessment of tumor growth every 2 days for two weeks and the size of tumor was measured with a ruler and recorded by length and width in centimeters. The volume of the tumor by a formula: V = 1/2ab².