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Cells and cell culture

AN Anne Cathrine Nøhr
MS Mohamed A. Shehata
DP Daniel Palmer
RP Rina Pokhrel
MV Maria Vallianou
SF Simon R. Foster
PG Patrick R. Gentry
DG David E. Gloriam
HB Hans Bräuner-Osborne
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All compounds were tested on a CHO-k1 cell line stably expressing GPR139 (CHO-GPR139)11 kindly provided by H. Lundbeck A/S, Denmark. GPR139-specific responses were assessed using a CHO-k1 cell line stably expressing the muscarinic acetylcholine receptor M1 (CHO-M1) (The Missouri S&T cDNA Resource Center, # CEM100TN00). The CHO-GPR139 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) F12-Kaighn’s (ThermoFisher Scientific # 21127) supplemented with 10% dialyzed fetal bovine serum (United States origin; ThermoFisher Scientific # 26400), 1% GlutaMAX-I (100X) (ThermoFisher Scientific # 35050061), and 100 units/mL penicillin and 100 µg/mL streptomycin (ThermoFisher Scientific # 15140122) and 1 mg/mL geneticin (ThermoFisher Scientific # 11811031). The CHO-M1 cells were grown in Ham’s F12 (ThermoFisher Scientific # 21765) supplemented with 10% fetal bovine serum (South American origin; ThermoFisher Scientific # 10270) + 100 units/mL penicillin and streptomycin and 0.25 mg/mL geneticin.

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