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Cell viability assay

XX Xuezhen Xu
OK Ok-Kyoung Kwon
IS In-Sik Shin
JM Jyotirling R. Mali
DH Dipesh S. Harmalkar
YL Yourim Lim
GL Gilhye Lee
QL Qili Lu
SO Sei-Ryang Oh
KA Kyung-Seop Ahn
HJ Hye-Gwang Jeong
KL Kyeong Lee
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We used colorimetric MTT assays to study the effects of benzofuran derivatives on cell proliferation. Cells (1 × 104 cells/well) were seeded onto a 96-well plate and treated with various concentrations of the compounds for 24 h. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, Amresco, OH, USA) was dissolved in PBS at 5 mg/ml then added to the well plate and incubated for 4 h at 37 °C. The supernatant was suctioned, and formazan crystals were dissolved in 100 μl DMSO. Optical density was measured using a microplate reader (SPARK10, TECAN, Switzerland) at 570 nm and cell viability was expressed as the proportion of untreated cells.

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