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In vitro, the cells were irradiated with the indicated doses using the X-ray irradiator MBR-1520R-3 (HITACHI Engineering & Services Co., Ltd., Tokyo, Japan). In vivo, the tumor was irradiated (5 Gy) using the above-mentioned X-ray irradiator. The mice were immobilized in a customized harness allowing exposure of the right flank, whereas the remainder of the body was shielded by an X-ray protector (HAGOROMO; Maeda Co., Tokyo, Japan).

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