Subcellular Localization Prediction

A new fasta file containing the sequences predicted by SignalP v4.1 was generated and input to the TargetP v1.1 server² for cell localization prediction. Inputting sequences were according to the instruction (Emanuelsson et al., 2000). The credibility partition is determined by the RC value, which is the difference score (diff) of two consecutive highest values; the higher the RC value level is, the lower the confidence of the data is. This study retained data located at “S” (containing SP) with RC values of 1, 2, and 3, and “M” (containing mitochondrial transit peptide mTP) with RC values of 4 and 5 for predicting protein-containing signal peptide sequences and secreting them extracellularly.