All animal studies were carried out according to the protocol approved by the Animal and Care Committee at the National Institute of Arthritis and Musculoskeletal and Skin Diseases (protocol # A019-02-01) and by the University of Miami Institutional Animal Care and Use Committee (protocol # 18–053). CD1 mice were purchased from Charles River, USA (Strain number: 022). Db/db mice were purchased from Jackson Laboratory. Both male and female mice were used in the studies. Treatments were performed at 7–9 weeks of age and all experiments were conducted using littermate controls.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.