Cisplatin induced acute kidney injury

Acute kidney injury was induced in mice by a single intraperitoneal injection of cisplatin (20 mg/kg body weight). Renal function was determined by measuring BUN and serum creatinine prior to the cisplatin injection and at 24h, 48h and 72h after injection. We used IL10^flox/flox injected with cisplatin as a control group and compared with IL10^flox/floxCD11c-Cre and IL10^flox/flox Foxp3^YFP-Cre mice injected with cisplatin. Based on previous studies, we included at least 5 mice in each group for cisplatin induced acute kidney injury experiments. Because of the variability of the litter size, we used different numbers of mice for each experiment. We performed concurrent controls within each experiment and only compare the experimental groups with the corresponding control group. After 72h of cisplatin injection mice were sacrificed by isoflurane anesthesia followed by cervical dislocation.