Transwell permeable supports (24 well, 8 µm pore size, Corning, USA) were used to measure the invasion capability of TNBC cells. After the upper chamber was coated at 37℃ with 200 µl of diluted BD Matrigel™ Basement membrane Matrix (BD, USA) gel solution for 4 h, 800 µl DMEM supplemented with 10% FBS was added to the lower chamber. Then, the upper chamber was seeded with 1 × 104 cells in serum-free medium, and the cells were incubated at 37℃ for 24 h. Subsequently, the cells were fixed in 4% paraformaldehyde and stained with 1% crystal violet, after which cotton swabs were used to gently remove the cells remaining on the upper membrane. We counted stained cells under a microscope and assessed cell invasion from five random fields.
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