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4.8. Superoxide Dismutase Activity Assay
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The activity of superoxide dismutase (SOD) in mice brain and liver homogenates was determined according to the inhibition of nitroblue tetrazolium reduction rate in the non-enzymatic phenazine methosulfate-nicotinamide adenine dinucleotide system assessed spectrophotometrically at 540 nm [70]. The SOD activity was expressed as U/mg protein, where U was the relative unit of activity defined as the amount of SOD required for the inhibition of nitroblue tetrazolium reduction by 50%.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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