ALDH Activity Assay

AS Alex Steimle
LM Lena Michaelis
FL Flaviana Di Lorenzo
TK Thorsten Kliem
TM Tobias Münzner
JM Jan Kevin Maerz
AS Andrea Schäfer
AL Anna Lange
RP Raphael Parusel
KG Kerstin Gronbach
KF Kerstin Fuchs
AS Alba Silipo
Hasan Halit Öz
BP Bernd J. Pichler
IA Ingo B. Autenrieth
AM Antonio Molinaro
JF Julia-Stefanie Frick
request Request a Protocol
ask Ask a question
Favorite

Intracellular ALDH activity in BMDCs was assayed using the Aldefluor kit (STEMCELL Technologies), according to the manufacturer’s instructions. Samples treated with the specific ALDH inhibitor diethylaminobenzaldehyde (DEAB) served for the determination of baseline fluorescence and defining ALDH-positive cells (Figure S5).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A