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For NLRP3 inflammasome activation, cells were primed by incubation with LPS (1 μg/ml) at 37°C for 3 h. Then, NLRP3 inflammasomes were activated by incubation of cells with nigericin (2.5 μM, denoted LPS + Nig) or ATP (5 mM, denoted LPS + ATP) for the indicated times.
Copyright and License information: ©2020 Ming, Zeng, Guo, Zhang, Li, Ma, Zhai, Chang, Yang, Wang, Yang and Chu.
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