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L6 cells were obtained from ATCC and cultured as described (Dolinar et al., 2018). Briefly, L6 cells were cultured in MEMα supplemented with 10% (v/v) FBS, 1% (v/v) pen strep, and 0.3% (v/v) fungizone. All experiments with L6 muscle cells were performed on the differentiated myotubes. To differentiate myoblasts into myotubes, myoblasts were grown in the presence of 10% (v/v) FBS until they were almost confluent and then for additional 7–9 days in MEMα with 2% (v/v) FBS.
Copyright and License information: ©2020 Pirkmajer, Bezjak, Matkovič, Dolinar, Jiang, Miš, Gros, Milovanova, Pirkmajer, Marš, Kapilevich and Chibalin.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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