Apoptosis Assay Using Annexin V-FITC Staining by Flow Cytometry

Apoptosis assay was carried out using an annexin V-FITC apoptosis detection kit by a FACSCalibur flow cytometer. Briefly, cells were seeded in 6-well plates for 24 h. The cells were incubated with the treatments (30, 100 μg/mL) for 6 h. The cells were then carefully washed with PBS and resuspended in PBS buffer. After that, the cells were incubated with annexin V-FITC for 30 min at 37°C, followed by flow cytometry analysis. Data were analyzed using the BD Cell Quest software. Percentage of live and apoptotic cells was determined using negative controls, prepared by staining control experiments with propidium iodide (PI) or annexin V.