SIRT1 Deacetylase Activity Assay

Having been divided into 4 groups (in the presence or absence of 10 mM drug HYA, the BV2 cells were, respectively, exposed to PBS, LPS, LPS+M or LPS+H), the total protein of BV2 cells was extracted by using a Protein Extraction Kit (Solarbio, HAOMA BioTECH, Guangzhou, China) as recommended by the manufacturer. Then the deacetylase activity of SIRT1 in total protein of BV2 cells was detected with a SIRT1 activity assay kit (Abcam, ab156065, UK) based on instructions of the manufacturer. Measurements of fluorescence intensity was carried out at 340 nm excitation and 460 nm emission using a microtiter plate fluorometer.