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Cells were plated in 6-well plates at 200 cells per well. After 1 day, they were incubated in the presence or absence of the indicated concentration of TKIs gefitinib and afatinib. Fresh media containing each TKI was added every 3 days. After 10 days, cells were fixed in methanol and stained with 0.5% crystal violet. The number of foci >1 mm was automatically determined using Image J software.
Copyright and License information: ©2020 Tsukumo et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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