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HDFs (5 × 104) were seeded per well in 6-well plates and cultured for 24 h. The following procedures were conducted as same as cell proliferation assay. But after treatment with exosomes, the cells were fixed and stained using a senescence-associated β-galactosidase (SA-β-gal) staining kit (Cell Signaling Technology, USA) according to the product instruction. Four images were captured in each well in four directions (up, down, left and right) using an inverted light microscope (Eclipse Ts2, Nikon, Japan), and senescent cells were counted by blue staining.
Copyright and License information: ©2020 Zhang et al.
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