3.3. Expression of the Recombinant Maltose Binding Protein-protein A (MBP-SPA) Fusion Protein

Masoud Reza Seyfi Abad Shapouri; Pezhman Mahmoodi; Dariush Gharibi; Masoud Ghorbanpoor; Sara Yaghoubi; Elham Rezaei; Mohammad Rashno; Neda Mehravar

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3.3. Expression of the Recombinant Maltose Binding Protein-protein A (MBP-SPA) Fusion Protein

MS Masoud Reza Seyfi Abad Shapouri

PM Pezhman Mahmoodi

DG Dariush Gharibi

MG Masoud Ghorbanpoor

SY Sara Yaghoubi

ER Elham Rezaei

MR Mohammad Rashno

NM Neda Mehravar

This method is extracted from research article: Iran J Biotechnol, Dec 2016

Molecular Cloning, Expression and Peroxidase Conjugation of Staphylococcus aureus Protein A

DOI: 10.15171/ijb.1267

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Bacterial colons, which contained recombinant plasmids were cultured in LB broth enriched with 20 mM glucose until OD₆₀₀ reached 0.5. Thereafter, IPTG (Isopropyl-β-D-thiogalactopyranoside) was added to the cultures followed by cultivation for another 4 h. Expression of the recombinant protein (~67 kDa) was examined by SDS-PAGE.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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